Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

Methods: We designed primers specific to the orf1ab and S genes of SARS-CoV-2. Total viral RNA was extracted using the QIAamp Viral RNA Mini Kit. Assa...

SARS-CoV-2 RNA in plasma samples of COVID-19 affected individuals: a cross-sectional proof-of-concept study

Plasma SARS-CoV-2 RNA seems to be associated with a worse COVID-19 outcome. Viremic patients were more frequently affected by hematological malignanci...

The utility of chest computed tomography (CT) and RT-PCR screening of asymptomatic patients for SARS-CoV-2 prior to semiurgent or urgent hospital procedures

In total, 625 patients were screened for SARS-CoV-2 using a combination of phone questionnaires, RT-PCR and chest computed tomography. Of the 625 pati...

Evaluation of the RealStar® SARS-CoV-2 RT-PCR kit RUO performances and limit of detection

Assessment of the RealStar® SARS-CoV-2 RT-PCR Kit Research Use Only (Altona) compared with the WHO-PCr. Assay showed a slightly higher sensitivity wit...

SARS-CoV-2 seroprevalence worldwide: a systematic review and meta-analysis

SARS-CoV-2 has been arguably the most important public health concern worldwide in 2020. We used a random-effects model to estimate pooled seroprevale...

Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA

IgG response was studied in relation to duration and severity of illness. The igG detection rate was higher in patients with severe disease. The BPL i...

Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal Swabs

Quantitative reverse transcription-PCR (RT-qPCR) is the preferred methodology for viral RNA detection. This study shows that RT-ddPCR allows the detec...

Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients

In total, 35/124 (26.6%) samples were RT-PCR positive, with 33/35 positive by NPS. A third or more of pure saliva samples from symptomatic patients we...

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