Variability of cycle threshold values in an external quality assessment scheme for detection of the SARS-CoV-2 virus genome by RT-PCR
Results of SARS-CoV-2 specific real-time reverse transcription (RT) are used for initial diagnosis and follow-up of Covid-19 patients and asymptomatic virus carriers. Clinical decision-making and health management policies often are based additionally on cycle threshold ( Ct) values to guide patient care. This study highlights the limitations of interpreting Ct values from the various SARS.-CoV genome detection protocols.

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